摘要：采用高效液相色谱法测定大鼠体内8-氯腺苷及其代谢物8-氯肌苷和8-氯腺嘌呤的浓度。试样用乙腈沉淀蛋白后，水层进样，采用YWG-C18。柱，以甲醇-1％醋酸(含0．1％四丁基溴化铵)为流动相，检测波长263nm。8-氯腺苷及其代谢物分离良好，最低检测浓度8-氯腺苷、8-氯肌苷和8-氯腺嘌呤分别为0．10μg/ml，0．50μg/ml，0．25μg/ml，日内精密度和日间精密度均在2．3％～16．0％，三种化合物回收率为70％～99％。

分类号：R917文献标识码：A

Determination of 8-chloro-adenosine and its metabolites in rats by HPLC

dou Guifang(Beijing Medical University，National laboratory of Nature and Biomimic Drug，Beijing l00083)

lou Yaqing,Sun Lili(tory of Nature and Biomimic Drug，Beijing l00083)

zhang Weiqing,Zou Anqing(Beijing Medical University，National laboratory of Nature and Biomimic Drug，Beijing l00083)

Abstract：To determine the concentration of 8-chloro-adenosine and its metabolites in rats with HPLC．The analytical column(250mm×4．6mm)was packed with 10μm YWG-C18H37．The mobile phase was a mixture of methanol and l％acetic acid(containing 0．1％tetrabutyl ammonium bromide)(20:80，V/V)．Detection was performed at 263nm.The 8-chloro-adenosine and its two metabolites were separated very well．The minimum detectable concentrations of 8-chloro-adenosine， 8-chloro-adenine and 8-chloro-inosine were 0．1μg/ml，0．25μg/ml and 0．50μg/ml respectively．The precisions within-day and day to day ranged from 2．3％to 16．0％．The mean recoveries ranged from 70％ to 99％．

Keywords：8-chloro-adenosine;8-chloro-adenine;8-chloro-inosineo; HPLC;pharmacokinetics基金项目：为国家生命科学中心1035资助项目(96005)参考文献：

［1］方家椿，石永进，彭静，等．8-氯腺苷的抗肿瘤作用研究．中华肿瘤杂志，1995，17(9)，48

［2］王宪焘，郑德先，刘睿，等．8-氯腺苷诱导MOLT-4细胞凋亡．科学通报，1997，42(2)，189

［3］詹京辉，方家椿，石永进，等．8=氯腺苷对小鼠肝癌H22细胞的抑瘤作用研究．中国药理学通报，1996，12(1)，71

［4］彭静，刘影，方家椿，等．新抗癌药8-氯腺苷的毒理学研究．中国药理学通报，1996，12(6)，548

收稿日期：1999-12-23