目的：本研究应用实验性家兔动脉粥样硬化后血管狭窄模型，经球囊血管成形术后，观察应用血脂康对血管内膜增生及内皮功能保护的作用。

方法：日本纯种雄性大耳白兔36只，体重2.5±0.5 kg,月龄6～8个月，予1.5 g/(kg·d)高胆固醇饮食喂养4周后，穿刺右股动脉并导入球囊导管至肾动脉下方，充盈球囊剥脱动脉内皮。继续高脂喂养4周，经左股动脉插管，行腹主动脉造影，对狭窄部位行血管成形术，再经造影证实扩张满意为止。共成功建立30只家兔动脉粥样硬化后血管成形术模型，随机分为对照组、高脂组和治疗组［血脂康0.8 g/(kg·d),血脂康为北大维信公司生产］；继续喂养4周后气栓处死。实验前后检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、血浆内皮素-1(ET-1)和血清一氧化氮(NO)。动物处死后，截取成形术部位血管标本，行弹力纤维染色，利用计算机图像分析系统分别测出新生内膜面积(NEA)、中膜面积(MA)和管腔面积(SA)，并计算NEA/MA的比率。

结果：所有3组TC、TG、LDL-C、ET-1高脂喂养4周后均明显升高(P＜0.05),而NO呈下降趋势；12周后，TC、TG、LDL-C、ET-1在治疗组中下降，低于第8周水平(P＜0.05),在高脂组中保持增高，与对照组比P＜0.05；治疗组在12周后明显下降，低于第8周水平，与对照组相比明显下降(P＜0.05),而NO水平则明显升高，与对照组比较P＜0.05。病理形态学观察NEA、NEA/MA比值的改变为：血脂康治疗组＜对照组，对照组＜高脂组，组间比较P＜0.05。

结论：血脂康具有抑制家兔血管成形术后内膜增殖和保护内皮功能的作用。

The effect of Xuezhikang on Neointimal Proliferation and Endothelial Function after angioplasty in Rabbits (Abstract)

Department of cardiology, The First Affiliated Hospital of China Medical University, Shenyang(110001), Liaoning

qi Guoxian, Zeng Dingyin, Liu Li, et al.

objective: To investigate the effects of Xuezhikang (XZhK, a Chinese patent medicine used for blood-lipid lowering) on the neointimal proliferation and endothelial function after angioplasty of the modelled rabbits.

Methods: angioplasty was performed on 30 modelled rabbits of atherosclerotic stenosis in celiac artery. After being fed with cholesterol-supplemented diet for 8 weeks, they were then randomly divided into the control group, high-cholesterol group and XZhK group. After an additional high-cholesteroldiet for 4 weeks, the local veassels were collected for morphological observation, and blood lipid, nitric oxide (NO) and endothelin-1 (ET-1) were also measured.

Results: the morphological observation showed that the neointimal area in the XZhK group was less than those in control group (p＜0.05), and that in control group was less than in high-cholesterol group (p＜0.05). The serum total cholesterol (TC)、 triglyceride (TG) and ET-1 levels were increased and the serum NO level was decreased after high-cholesterol diet. however, as compared with the control group and high-cholesterol group, there was more remarkable decrease in TC、 TG levels and increase in NO level in XZhK group.

Conclusion: xZhK had the effects of inhibiting the neointimal proliferation and of protecting the endothelial function after angioplasty in rabbits.