摘要：目的探讨白细胞介素13(IL-13)基因在原发性非IgA系膜增生性肾炎(MsPGN)、原发性局灶节段性肾小球硬化症(FSGS)和狼疮肾炎(LN)患者肾组织的表达及其意义。方法采用原位杂交技术(ISH)检测34例上述3种肾脏病患者及4例对照组肾组织中IL-13mRNA表达量的变化，分析IL-13基因表达量与肾功能变化的关系。结果IL-13mRNA在FSGS、MsPGN和LN患者肾组织内表达均增高，而对照者肾组织内无明显IL-13mRNA表达。LN患者肾小管间质区IL-13mBNA表达水平与狼疮活动相关，MsPGN及FSGS患者肾小管间质区IL-13mRNA表达量与肾功能变化及24h尿蛋白量相关。结论IL-13可能直接参与人类肾小球疾病的免疫反应过程。

Interleukin-13 gene expression in renal tissne of human kidney diseases

ZHANG Zhao ,JIANG Liming ,YE Feng,et al.

(Institute of Nephrology,Affiliated Hospital of Guangdong Medical College,Guangdong Zhanjiang 524001,China)

Abstract：Objective To study the clinical significance of interleukin- 13 (IL-13) gene expression in renal tissue of human kidney diseases.Methods 4 controls and 34 patients with primary focal segmental glomerular sclerosis(FSGS),non-IgA primary mesangioproliferative glomerulonephritis(MsPGN) and lupus nephritis(LN) were included in this study.IL-13mRNA expression in renal tissue was detected by in situ hybridization(ISH) technique.Results Up-regulating expressions of IL-13mRNA in the renal tissues of FSGS,MsPGN and LN were found,whereas little expression of IL-13mRNA in the renal tissue of the controls was observed.The elevated expression of IL-13mRNA in tubulointerstitium was closely related to lupus activity in LN.The levels of IL-13mRNA in tubulointerstitium correlated to the renal function and urinary albumin excretion in FSGS and MsPGN.Conclusion IL-13 may be involved in the immune reaction of renal tissue in human kidney diseases.

Keywords：Interleukin-13;Gene expression;Lupus nephritis;Glomerular sclerosing,focal;Mesangioproliferative glomerulonephritis

基金项目：广东省重点科学攻关项目基金资助项目(199556)

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收稿日期：2000-02-15