摘要：目的 了解狼疮肾炎(LN)肾组织中核因子(NP)-κB的表达并探讨其与LN肾脏病理改变和肾功能损害的关系。方法以NF-κB亚基P65单抗为抗体，采用微波免疫组织化学染色(APAAP法)检测LN肾组织NF-κB表达，并进一步分析其与肾小球内C-myc蛋白表达、LN活动指数、肾脏病理和功能损害的关系。结果狼疮肾炎肾组织中NF-κB表达较正常肾组织显著增高，以WHOⅣ型为最显著。NF-κB在肾小球和肾小管均有表达，但小管表达更显著。LN肾组织NF-κB阳性细胞数与肾小球C-myc蛋白表达量、肾组织活动指数、肾脏病理改变和肾功能损害显著相关。结论NF-κB可能参与了LN的发病机制，肾组织中NF-κB的表达可作为反映狼疮肾组织活动病变和进行性肾损害的参考指标。

Expression of transcription of NF-κB in renal tissue of lupus nephritis

XU Hanshi ,YE Rengao ,ZHANG Shihong

(Department of Nephrology, First Affiliated Hospital,Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China)

Abstract：Objective To investigate the role of NF-κB in the pathogenesis of lupus nephritis(LN). Methods A microwave-based immunohistochemistry (APAAP) was used to detect the expressioh of NF-κB p65 and C-myc protein in renal tissue of LN and normal control.At the same time,the correlation between expression of NF-κB and renal injuries in LN was examined.Results The expression of NF-κB in LN was higher than that of normal control,the highest one was type Ⅳ LN.The number of positive cells expressing NF-κB in renal tissue of LN showed a strong correlation with C-myc protein expression, kidney activity of LN, histological changes and functional lesion in LN, respectively. Conclusions NF-κB may play an important role in the pathogenesis of LN,and the number of positive cells expressing NF-κB may be considered as a predictor for the kidney activity and progressive renal injury in LN.

Keywords：Lupus nephritis; Nuclear factor-κB

基金项目：中山医科大学211工程课题资助项目(98150)

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收稿日期：1999-06-01