摘要：目的研究肾综合征出血热病毒(HFRSV)对肾小管上皮细胞(RTC)的致病作用。方法应用HFRSV感染体外培养的人胚RTC(HFRTC)，采用原位分子杂交技术检测HFRTC内的HFRSV并作台盼蓝染色、透射电镜(TEM)观察。结果(1)HPRSV能够直接感染培养的HFRTC；(2)从第7天起被感染的HPRTC死亡率明显高于对照组；(3)TEM观察表明HFRSV可致HPRTC的细胞膜、细胞器损害。结论HFRSV能够直接损害体外培养的HPRTC，其对RTC的损害作用可能直接参予肾综出血热(HPRS)的发病。

The study of cytopathogenic effect of hemorrhagic fever with renal syndrome virus on renal tubular cells

yAO Lijun,DENG Anguo,HUANG Pei(Department of Nephrology, Union Hospital , Tongji Medical University, Wuhan 430022, China)

Abstract：Objective To study the cytopathogcnic effect of epidemic hemorrhagic fever with renal syndrome virus(HFRSV) on renal tubular cells(RTC) . Methods Human fetal renal tubular cells(HFRTC) were cultured in vitro. HFRTC infected or not infected by HFRSV were observed by using trypan-blue stain and transmission electron microscopy(TEM) . Viral-mRNA was detected by in situ molecular hybridization. Results (1)HFRSV could directly infected hFRTC; (2)The death rate of HFRTC in the infection group was significantly higher than that in the control group 1 to 4 weeks after infection; (3) Injuries of cell membrane and cell organs after infection with HFRSV were significantly earlier and more severe as compared to control by means of TEM. Conclusion HFRSV can directly damage renal tubular cells(RTC), which contributes to the pathogenesis of hemorrhagic fever with renal syndrome(HFRS).

Keywords：Hemorrhagic fever; Renal syndrome; Renal tubular cells; Hemorrhagic fever virus基金项目：卫生部资助基金项目(96-1-142)

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收稿日期：1999-09-23