摘要：目的进一步探讨晚期糖基化终产物修饰的β2微球蛋白(AGE-β2m)对关节固有细胞的生物学作用，确定人类关节滑膜细胞是否表达对AGE特异的受体。方法分离、培养人关节A型和B型滑膜细胞，用免疫组织化学法及流式细胞仪法分别观察滑膜细胞表面AGE受体1(AGE-R1)、AGE受体2(AGE-R2)、AGE受体3(ACE-R3)及AGE受体(RAGE)的表达，用逆转录－聚合酶链反应(RT-PCR)技术检测滑膜细胞内各种AGE受体的mRNA水平。结果A、B两型细胞均表达RAGE和AGE-R3，两种受体均分布于细胞表面，RT-PCR示滑膜A、B两型细胞内存在RAGE和AGE-R3mRNA。两型滑膜细胞均不表达AGE-R1和AGE-R2。结论人类关节滑膜A、B两型细胞表达RAGE和AGE-R3，提示这类关节固有细胞参与了AGE的代谢，透析相关性淀粉样变时也可能成为AGE病理生物效应的靶细胞。

Expression of advanced glycation end products receptors on human joint synovial cells

hOU Fanfan,JIANG Jianping,ZHANG Xun(Division of Nephrology , Nanfang Hospital , Guangzhou 510515, China)

Abstract：Objective To determine the expression of advanced glycation end products(AGE) binding proteins on human joint synovial cells for elucidating the pathobiological effects of β2m modified with AGE(AGE-β2m) on joint resident cells. Methods Type A and type B synovial cells were isolated and cultured in vitro. The expression of AGE receptor 1 (AGE-R1), AGE receptor 2 (AGE-R2), AGE receptor 3 (AGE-R3) and 35 KD receptor for aGE(RAGE) on synoviocytes were detected by immunofluorescent staining using specific antibodies and flow cytometric analyses, mRNA of AGE receptors was examined by RT-PCR techniques. Results RAGE and AGE-R3, but not AGE-R1 and AGE-R2, were constitutively expressed on the membrane surface of both type A and type B synovial cells. These two types of synovial cell also expressed mRNA of RAGE and AGE-R3. Conclusion Human joint synovial cells express specific AGE binding proteins, RAGE and AGE-R3, suggesting that these cells may be involved in AGE metabolism and might be the target of the biological effects of AGEs in dialysis-related amyloidosis.

Keywords：Synovial cells; Advanced glycation end products; Receptor基金项目：国家自然科学基金资助项目(39970341)参考文献：

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收稿日期：2000-01-08