摘要：目的探讨不同剂量的川芎嗪对高糖透析液作用下慢性大鼠腹膜透析(腹透)模型腹膜间皮细胞的形态和功能的影响及它们之间的关系。方法40只SD大鼠随机分为4.25％腹透液(HG组)、4.25％腹透液＋40mg／L川芎嗪(HGL组)、4.25％腹透液＋160mg／L川芎嗪(HGH组)、对照组。除对照组外，余3组每天分别腹腔内注入20ml含不同剂量川芎嗪的4.25％透析液[0(HG)、40mg／L(HGL)、160mg／L(HGH)]。8周后进行腹膜功能试验，同时对细胞印片进行形态学分析。结果与HGH和HG组相比，HGL组腹腔内液体量和净超滤量显著增高(P＜0.01)，透出液与血浆尿素浓度比(D／Purea)显著降低，4h透出液／透析液葡萄糖(D／D0)值和尿素清除率(Curea)显著增高。但总重吸收率和直接淋巴吸收率在各透析组之间无显著差异。细胞印片上间皮细胞的细胞密度在HGL组比HGH组和HG组显著增加，而表面积显著减少。低剂量川芎嗪显著提高腹膜超滤量与间皮细胞表面积减少密切相关(r=-0.944，P＜0.05)。结论低剂量川芎嗪加入高糖透析液中长期应用可显著提高腹膜超滤量，这一作用可能与其减轻腹膜间皮细胞的肥大有关。

Effects of ligustrazine on the peritoneal membrane function and the mesothelial cell morphology of chronic peritoneal dialysis rats

YANG Qiongqiong ,YE Rengao ,YANG Xiao

(Department of Nephology, Key Clinical Kidney Research Institute of Ministry of Health , First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China)

Abstract：Objective To investigate the influence of different concentrations of ligustrazine on the cell morphology and the peritoneal membrane function and their relationship in chronic peritoneal dialysis rats with infusion of high glucose dialysate. Methods 40 SD rats were divided randomly into 4 groups. Except control group, other groups daily received infusion of 20 ml 4.25% glucose dialysate containing different concentrations of ligustrazine [0 (HG), 40 mg/L (HGL), 160 mg/L (HGH)] respectively for 8 weeks. The peritoneal membrane function was investigated by peritoneal transport test , and the rat peritoneal mesothelial cells(PMCs)morphology was analyzed from the cell imprints. Results Compared to the HG and HGH groups, the intraperitoneal volume and net ultrafiltration in HGL group were significantly higher ( P＜ 0.01), D/Purea significantly lower, and D/D0 and Curea after 4 hours of dialysis significantly higher. The peritoneal fluid absorption rate as well as direct lymphatic absorption rate had no significant difference between all the groups. The density of cell population from analysis of cell imprints in HGL group was significantly higher than that of HGH and HG groups, but the mean surface area was significantly lower. Long-term use of low dose ligustrazine in the high glucose dialysate could significantly increase the ultrafiltration volume, which was significantly correlated to the decreasing of cell surface area (r=-0.944, P＜0.05) of PMCs. Conclusion Low-dose ligustrazine added to high glucose dialysate can significantly increase the ultrafiltration volume, which might related to the decrease of the hypertrophy of PMCs.

Keywords：Peritoneal dialysis; Ligustrazine; Adequacy of dialysis; Peritoneal mesothelial cells

基金项目：卫生部临床重点攻关资助项目(97040228)

参考文献：

［1］Schreiber M. Membraoe viability in the long-term peritoneal dialysis patients. Petit Dial lnt, 1997, 17(S3): 19-24.

［2］陆才生，叶任高，孙林，等．含川芎嗪腹膜透析液对腹膜透析效能的影响．中国中西医杂志．1998．18：709-711

［3］Tao Wang. Olof Heimburger, Hui-hcmg Cheng, el al. Effect of increases dialvsate fill volume on peritoneal fluid and solute transport. Kidney Int , 1997, 52: 1068-1076.

［4］Gntloib L. Shostak A, Wajsbist V. et al. High glueose iodueed a hypertrophic, senescent mesothelial cell phenotype after long in vivo exposure. Nephron 1999. 82: 164-173.

［5］徐根兴主编．定量细胞学和细胞化学技术．第一版．吉林：吉林科技出版社．1993：17-79．

［6］肖静．川芎嗪研究的新进展．华西药学杂志．1993．8：170-172．

［7］Krediet RT. The peritoneal membrane in chronic peritoneal dialysis. Kidnev Int, 1999. 55: 341-356.

［8］Di Palo N. Garosi G, Petrini G. et al. Morphologieal and morphometric changes in mesothelial cells during peritoneal dialysis in the rabbit. Nephron, 1996, 74: 594-599

［9］Yamamoto T. lzumotani T, Otoshi T. et al. Morphological studies of mesothelial cells in CAPD effluent and their clinical signifieanee. Am J Kidney Dis, 1998, 32: 946-952.

［10］Churchill DN, Taylor DW, Keshaviah PR, the CANUSA Peritoneal Dialysis Study Group. Adequacy of dialysis and nutrition in eontinuous peritoneal dialysis : association with clinical outcomes. J Am Soc Nephrol, 1996, 7: 198-207.

收稿日期：2000-03-08