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正常大鼠皮肤肥大细胞形态学观察及所含生物活性介质的测

2022-07-29
来源:求医网
摘要用体重200克左右的正常大鼠20只,在腰背部两侧对称部位取皮肤标本,制常规组织切片,相邻切片分别用琉董及阿尔新兰染色,光镜下观察细胞形态及分布,用图象分析仪测量细胞着色面积和胞浆内颗粒所含活性介质相对含量。结果:肥大细胞是三层分布,浅筋膜分布最多。定量分析提示肥大细胞中肝素含量大于组织胺含量。

The Measurement of Mast Cell and Its Active Material in Skin of Normal Rat

Li Ji, Hu gang, Bai shuling, et al

(Department of Anatomy, China Medical University, Shenyang 110001)

AbstractTwenty Wistar rats were used to obtain the quantitative data of mast cells and its activity materiae in the normal rat skin. The samples were taken from the back of rats on two sides. The tissue slices were stained by thionin and alcian blue. the histological methods was used, and the morphological characteristics of the mast cells in the skin of the normal rats back were observed. Mast cells in the papillary layer, in the reticular layer and in the hypodermis of rat skin were observed. It is shown that not only the mast cells mainly olcated in the hypodermis but also the content of heparin is much more than histamine in mast cells.

Key words:mast cell;rat;heparin;histamine

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