摘要本实验利用放线菌酮建造细胞凋亡动物模型，用原位末端标记和电镜方法观察了大鼠肠系膜淋巴结淋巴细胞凋亡的形态学变化。结果显示放线菌酮处理4 h后，肠系膜淋巴结部分淋巴细胞处于凋亡状态，主要表现为细胞核凝聚, 染色质边集，可见线粒体、内质网增殖, 自噬体和凋亡小体形成。结果表明放线菌酮主要诱发肠系膜淋巴结生发中心的B淋巴细胞凋亡，线粒体、内质网的增殖是细胞凋亡的一种主动行为。

Cycloheximide-induced Apoptosis of Lymphocyte in Mesenteric Lymph Nodes

Huo Xia,Piao Yingjie, Huang Xinxu

(Shantou University Medical College, Shantou, 515031)

Xu Xijin

(Central Lab, Dept. of Hist-Embryology

AbstractUsing the SD rats as an experimental model of apoptosis in lymphocytes of the mesenteric lymph nodes with cycloheximide, the structure of lymphocyte were observed with in situ end labeling and electron microscope technique. The results showed that many of the lymphocytes displayed the morphology of apoptosis after 4 hours treatment with cycloheximide with shrinkage of nuclear pyknosis, or chromatin condensation and margination, mitochondria and endoplastic reticula proliferation, autophagosomes and apoptotic bodies formation. Our present study demonstrated that cycloheximide mainly induced apoptosis of B lymphocyte in germinal center of mesenteric lymph nodes. The phenomenon of mitochondria and endoplastic reticula proliferation was an active process of apoptosis.

Key Wordscycloheximide； lymphocyte； apoptosis； in situ end labeling； electron microscope

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