摘要为了探讨降钙素基因相关肽CGRP对大鼠脑缺血再灌注后突触体素(synaptophysin, p38)表达的影响，我们采用暂时夹闭双侧颈总动脉法制作大鼠脑缺血再灌注模型，经右侧颈内动脉主入CGRP，采用单克隆抗p38抗体免疫组织化学染色及显微图像方法研究CGRP对大鼠再灌注海马内p38的影响。结果表明：大鼠缺血再灌海马内p38反应产物较正常组显著降低(P＜0.01)，而注射CGRP组阳性产物明显高于缺血再灌注组(P＜0.01)。这说明大鼠缺血再灌注p38表达明显减少，而CGRP可上调缺血再灌注p38的表达。本文结果提示：CGRP参与缺血神经元突触体素的调节，CGRP对缺血神经元有一定的修复作用。

Effect of CGRP on Synaptophysin in the Hippocampus of the Ischemic and Reperfusion Rat

Ding Xiaohui, Hua Fang, Liu Wenrui, Fang Xiubin

(Department of Neurobiology, China Medical University, Shenyang, 110001)

AbstractTo study the effect of CGRP on synaptophysin in the hippocampus of the ischemic and reperfusion rat, we established the model of ischemic reperfusion rat by tentatively clamping bilateral common carotid arteries. Immunohistochemical technique and microimage analysis were used to estimate the effect of CGRP on the expression of synaptophysin in ischemic neurons in hippocampus. The results showed that the expression of p38 was signifcantly decreased after cerebral ischemic and reperfusion, and CGRP increased the expression of p38. These results indicate that CGRP participates in the regulation of synaptophysin in ischemic neurons, has some recovery effect on ischemic neurons.

Key wordssynaptphysin; calcitonin gene-related peptide; neuron ischemia; hippocampus; rat

辽宁省自然科学基金资助项目(619019)

参考文献

1，Deshpande jK, Siesjo BK, Widoch T. Calcium accumulation and neuronal damage in the rat hippocampus following cerebral ischemia. J Cereb Blood Flow Metab, 1987,7(1):89

2，Holland JP, Syderff SGC, Taylor WAS, et al. Calcitonin gene-related peptide reduces brain injury in a rat model of focal cerebral ischemia. Stroke, 1994,25:2055

3，王福庄，刘振伟，要航，等.降钙素基因相关肽对缺氧时海马细胞内游离Ca²⁺的影响.中国应用生理学杂志，1994，10(4)：325

4，Jahn R, Schiebler W, Duimet C, et al. A 38000-dalton membrane protein (p38) present in synaptic vesicles. Proc Natl Acad Sci USA, 1985,82(12):4137

5，Thomas L, Hartung K, Langosh D, et al. Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane. Science, 1988,242(4881):1050

6，Alder J, Lu B, Valtorta F, et al. Calcium-dependent transmitter secretion reconstituted in xenopus oocytes: Requirement for synaptophysin. Scierce, 1992,257(5070):657

7，Thiel G. Synapsin Ⅰ, Synapsin Ⅱ, and synaptophysin: Marker proteins of synaptic vesicles. Brain Path, 1993,3(1):87

8，Vanlookeren Campagne M, Destreicher AB, Van Bergen Enhenegouwen PMP, et al. Ultrastructural double localization of B-50/GAP43 and synaptophysin (p38) in the neonatal and adult rat hippocampus. J Neurocytol, 1990,19(6):948

9，钱雪松，李陈莉，曹玉纯，等.突素体素.神经解剖学杂志，1997，13(3)：291

10，Wiedenmann B, Franke WW. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38000 characteristic of presynaptic vesicles. Cell, 1985,41(3):1017

11，Masliah E, Terry RD, Alford, et al. Quantitative immunohistochemistry of synaptophysin in human neocortex: an alternative method to estimate density of presynaptic terminals in paraffin sections. J Histochem Cytochem, 1990,38(6):837

12，Preibisz JJ. CGRP and regulation of human cardiovascular homeostasis. Am J hypertens, 1993,434