摘要：目的，为了证实TNF-α对局部脑缺血/再灌流区ICAM-1 mRNA表达的影响；方法：运用原位杂交技术对60只雄性SD大鼠进行了研究。结果：局部脑缺血/再灌流诱导脑微血管和毛细血管以及局部炎症细胞ICAM-1 mRNA的表达，其表达发生于脑缺血1h/再灌流2h，再灌流8h达到高峰。TNF-α明显的加强脑缺血区ICAM-1 mRNA的表达，其作用在脑缺血1h/再灌流2h至8h均较明显。再灌流4h，TNF-α的诱导作用最显著。结论：局部脑缺血/再灌流诱导ICAM-1 mRNA表达，TNF-α对其表达具有明显的促进作用。提示TNF-α通过调节ICAM-1 mRNA的表达参与脑缺血/再灌流损伤病理过程。

TNF-α Upregulates ICAM-1 mRNA Expression in Focal Cerebral lschemic Area in Rats

lei Wanlong(Department of Anatomy, Sun Yat-sen University of Medical Sciences, Guangzhou 510089)

liu Yong(Department of Anatomy, Xi’an Medical University, Xi’an)

yuan Ounfang(Department of Anatomy, Sun Yat-sen University of Medical Sciences, Guangzhou 510089)

Abstract：Objective:To test the effect of tNF-α on ICAM-1 mRNA expression in ischemic at rat. Method: In situ hybridization histochemistry was used in the study. Results: ① Focal ischemia/reperfusion-induced ICAM-1 mRNA expression occurred on the microvascular and capillary endothelial cells, and focal inflammatory cells at lh ischemia/2h reperfusion, peaked at 8h reperfusion. ② TNF-α induced strongly ICAM-1 mRNA expression in the ischemic cerebral area during 2h-8h reperfusion, and the strongest induce-effect was at the time point of 4h reperfusion. Conclusion: Focal cerebral ischemia/reperfusion induced ICAM-1 mRNA expression in the ischemic brain, and tNF-α accelerated ICAM-1 mRNA expression. This result indicated that TNF-α participated in the pathogenesis of ischemic cerebral injury.

Keywords：cerebral ischemia/reperfusion; ICAM-1; TNF-α参考文献：

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