摘要：本文采用2-VO方法对Wistar大白鼠制作血管性痴呆模型，经Morris迷宫实验检测其痴呆程度，通过透射电镜对轻度、中度和重度痴呆大鼠海马区神经元超微结构进行观察。结果显示，不同程度血管性痴呆时海马区神经元可见到不同程度的超微结构改变，其特征为神经元皱缩，细胞内胞质贫乏，核糖体、线粒体和内质网数量减少，胞浆内含有大量溶酶体和细胞丝，细胞核密度增高，有的染色体边聚成块，边界不规整，核仁与核膜溶解，细胞核膜内陷包裹核碎片形成凋亡小体。痴呆程度越重，超微结构改变越明显。

Ultrastructural Study on the Hippocampal Neurons of Vascular Dementia in Rats

zhao Xianlin,Li Dongpei,Fang Xiubin,Yang Guorui(Department of Neurosurgery, The First Clinical College, China Medical University, Shenyang, 110001)

Abstract：Wistar’s rats of experimental group were treated with a permanent bilateral occlusion of both common carotid arteries (2-VO)for establishing vascular dementia model. These models were checked by Morris maze and the ultrastructure of the hippocampal neurons with the light, middle and severe degrees of vascular dementia was observed by electron microscope compared with control group. The results showed that the changes of the ultrastructure were found among the hippocampal neurons of vascular dementia model. The character is that chromatin condensed and clumped in apoptotic nuclei and the nuclear membrane could be seen to break into apoptotic body. These results showed that the apoptosis of the hipoocampal neurons in the vascular dementia had intimate relationship with serious degree of the dementia.

Keywords：vascular dementia; animal model; ultrastructure ;rat基金项目：辽宁省自然科学基金资助项目NO．619019参考文献：

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