摘要：目的：研究葡萄糖、精氨酸对新生大鼠胰B细胞群的影响。方法：选用Wistar新生大鼠，自出生后第2日起腹腔注射葡萄糖加精氨酸，共6天。分别于出生后第10天、20天剖腹取胰腺，进行常规HE及免疫组织化学(PAP)染色，显示胰岛及B细胞。并结合图像分析仪测量胰岛及B细胞。并结合图像分析仪测量胰岛及B细胞的直径。结果：生后20天，实验组大鼠胰B细胞群较第10天的明显增大，而对照组B细胞群却较第10天显著缩小；第20天两组胰岛直径均较第10天的小，但以对照组的变化明显。结论：在葡萄糖、精氨酸的作用下，新生大鼠胰B细胞群及胰岛其它内分泌细胞增生。

Morphometric studies of pancreatic B-cells mass in neonatal rat after injection of glucose and arginine

ao Zhujun,Huang Yue,Liu Su,Li dirong(Department of Anatomy, China Medical University, Shenyang 110001)

Abstract：In order to study the effect of glucose and arginine on pancreatic B-cells mass, newborn rats were treated with glucose and arginine(G+A) for 6 days and were killed on the tenth and twentieth day after birth. The B-cells were sbowed by immunohischemistry (PAP method). The diameter of B-cells mass, quantified by the Medical Image Processing system,showed a definite increase in the twentieth day’s G+A treated rats, The B-cells mass of control rat, however, showed a decrease. In addition, the diameter of islet in two group rat had decrease, but that of control animals was significantly lower than that of G+A treated group. Thus, with the stimulation of g+A, the neonatal B-cells mass and non-B-cells mass have a greater capacity to growth.

Keywords：glucose, arginine, B-cells mass, neonatal rat, image analysis基金项目：国家自然科学基金资助项目(NO．39260031)

参考文献：

［1］Hole RL, Plan-Smith MCM, Sharp GWG. Development of the biphasic response to glucose in fetal and neonatal rat pancreas.Am J physiol, 1988, 254(1): E167

［2］Senecat O, Martignat L, Elmansour A, et al. Diabetes enhancement and increased islet antigen expression following neonatal injections of glucose and arginine in non- obese diabetic mice. Metabolism, 1994, 43(11): 1410

［3］Buschard K, Jorgnsen M, Anae K, et al. Prevention of diabetes mellitus in BB rats by neonatal stimulation of β cells. Lancet,1990, 335(8682), 134

［4］Susan BW, Deanna D, John L, et al. Compensatory growth of pancreatic β-cells in adult rats after short-term glucose infusion.Diabetes, 1989, 38:49

［5］黄跃，敖竹君,刘苏.新生大鼠胰B细胞的发育及葡萄糖、精氨酸对它的影响．贵州医药，1997，21(4)：201

［6］敖竹君，李维才，包含，等．葡萄糖、精氨酸对新生大鼠B细胞分泌颗粒影响的立体分析．解剖学报，1997，28(4)：407

［7］Woerner CA. Studies of the islands of largerhans after continuous injection of dextrose. Anat rec, 1938, 71 :33

［8］Diane TF, Luisa S, Susan BW. Dynamics of B-cells mass in the growing rat pancreas--Estimation with a simple mathematical model. Diabetes, 1995, 44:249