摘要：选用高特异性单克隆抗GLP-1-IR(7-36)酰胺抗体，用免疫组织化学方法估价了GLP-1-IR细胞在鼠、猪和人胎儿肠道中的分布。结果发现：在各段肠管都可见到GLP-1-IR细胞；GLP-1-IR细胞散在于肠腺的柱状上皮细胞之间，多位于肠腺的基底部，呈开放型内分泌细胞形态，即L细胞(EG细胞)；不同种属问GLP-1-IR细胞密度不同，但分布规律相同，即自小肠和大肠的近端向远端逐渐增大。结果表明，分泌GLP-1的L细胞(GLP-1-IR细胞)主要分布在肠道远端，这提示，远端肠道中大量存在的L细胞可以接受在近端胃肠道未被吸收的营养物质的刺激，使L细胞释放GLP-1入血。

Study of Distribution on GLP-1-IR Cells in the Gut of Rat, Pig and Foetus of Human

wang Junbo,Li Yanjun,Ning Shujun, et al(Department of Anatomy, Medical College of Jiamusi University, Juamusi 154002)

Abstract：We used a highly specific monoclonal antibody against GLP-1 (7-36) amide to evaluate the distribution of GLP -1-IR cells in the gut of rat, pig and foetus of human by immunohistochemistry. We found, GLP-1-IR cells in various guts of rat, pig and foetus of human with distribution scatteredly among the basal portion of intestinal glands. The cell shape corresponded with open type cells, i.e. L-cells(EG cells). The density of GLP-1-IR cells was different in races, but the distribution regularity of GLP-1-IR cells was same. It showed that a continuous increase from the proximal to the distal portion of small and bowel. The present results indicated that L-cells (GLP-1-IR cells) secreting GLP-1 presominantly located in the distal intestinal tract. It is suggest that large amounts of L-cells in the distal intestinal tract may receive stimulation of nutrients that haven’t been absorbed in the upper gastrointestinal tract and release GLP-1 circulation.

Keywords：glucagon-like peptide-1; GLP-1 immunoreactive; non-insulin-dependent debates mellitus参考文献：

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