摘要：目的探讨胎盘血管内皮生长因子（VEGF）在妊高征中的表达及其与胎盘释放前列环素（PGI₂）、血栓素A₂（TXA₂）的关系。方法分别采用蛋白免疫印渍（Westernblot）和放免技术测定25例孕晚期正常妊娠（NLP）及25例妊高征（PIH）患者的胎盘VEGF表达及胎盘和血浆中的PGI₂、TXA₂水平。结果与NLP比较，PIH组胎盘VEGF的表达显著降低（P＜0.01）；胎盘组织及血浆中PGI₂水平及PGI₂／TXA₂显著下降（P＜0.01），TXA2水平显著升高（P＜0.01）；PIH中胎盘VEGF表达与其产生的PGI₂、PGI₂／TXA₂呈显著正相关（P＜0.01），与TXA₂呈显著负相关（P＜0.01），并与临床收缩期血压（P＜0.01）和舒张期血压（P＜0.05）呈显著负相关。结论胎盘VEGF下降与妊高征患者血压升高密切相关，可能是通过下调胎盘及母血PGI₂／TXA₂参与了这一过程。

分类号：R714.24文献标识码：A

文章编号：1000-8861（2001）01-0043-04

Expression of vascular endothelial growth factor and its relationship with release of vascular active substances in placentae of patients with pregnancy induced hypertension

LIU Xia（Department of GynecologicandObstetric，Daping Hospital， the Third Military Medical College，Chongqing 400042，China）

LI Li（Department of GynecologicandObstetric，Daping Hospital， the Third Military Medical College，Chongqing 400042，China）

Abstract：ObjectiveTo investigate the expression of placental vascular endothelial growth factor （VEGF） and its relationship with placental release of prostacyclin I₂ （PGI₂） and thromboxane A₂（TXA₂） in patients with pregnancy induced hypertension （PIH）．Methods Twenty-five patients with PIH and twenty-five normal late pregnant （NLP） women in the third trimester were observed in this study. The expression of VEGF protein in placenta， the plasma and placental homogenate levels of PGI₂ and TXA₂ were determined by Western blot and radioimmunoassay respectively. Results Compared with NLP， the expression of placental VEGF protein was significantly decreased （P＜0.01）． Plasma and placental homogenate levels of PGI₂， PGI₂／TXA₂ in patients with PIH were significantly decreased （P＜0.01） while TXA₂ level was increased （P＜0.01）． There was a significant positive correlation between placental VEGF and PGI₂ and PGI₂／TXA₂ （P＜0.01）． A significant negative correlation was found between placental VEGF and TXA₂ （P＜0.01）， systolic （P＜0.01）and diastole blood pressure（P＜0.05）． Conclusion It is suggested that the reduction of placental VEGF may down-regulate local PGI₂／TXA₂， resulting in the imbalance of maternal PGI₂／TXA₂ and dysfunction of endothelial cell.

Keywords：pregnancy induced hypertension； vascular endothelial growth factor； placenta； prostacyclin I₂； thromboxane A₂

作者简介：刘霞（1971－），女，河北保定市人，住院医师，博士生，主要从事妊高征发病机制的研究。Tel： 023-68757731；E-mail：LiuXiaLx＠163．net参考文献：

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收稿日期：2000年9月22日

修稿日期：2000年11月6日

出版日期：2001年1月15日