摘要：目的构建表达乙型肝炎病毒（HBV）S蛋白的重组质粒pCR3.1-S作为核酸疫苗，观察重组人白细胞介素2（rhIL-2）作为佐剂对其诱导BALB／c小鼠产生免疫应答的影响。方法分别以ELISA法、³H-TdR掺入法、Cr⁵¹4h释放法等测定免疫小鼠的血清抗HBs、淋巴细胞增殖活性、淋巴细胞杀伤效应，初步研究不同免疫组体液和细胞免疫应答的差异。结果血清抗HBsELISA效价，rhIL-2组与对照组相比较差异不显著（P＞0.05）；淋巴细胞增殖指数、淋巴细胞杀伤率，1000U（只^．次）rhIL-2／组和2000U（只^．次）rhIL-2／组与对照组相比较差异显著（P＜0.05），而500U（只^．次）rhIL-2／组与对照组相比较无显著差异（P＞0.05）。结论一定剂量的rhIL-2作为佐剂可增强HBV核酸疫苗的免疫效应。

分类号：R512.6文献标识码：A

文章编号：1000-8861（2001）01-0027-03

Effects of different doses of interleukin 2 as adjuvants on immune responses induced by nucleic acid vaccines of hepatitis B virus

LI Wen-bo（Department of Gastroenterology， General Hospital of Jinan Military Region， Jinan， 250031，China）

YAO Zhi-qiang（Department of Infectious Diseases， Tangdu Hospital， the Fourth Military Medical University， Xi’an 710038， China）

ZHOU Yong-xing（Department of Infectious Diseases， Tangdu Hospital， the Fourth Military Medical University， Xi’an 710038， China）

QUAN Qi-zhen（Department of Gastroenterology， General Hospital of Jinan Military Region， Jinan， 250031，China）

FENG Zhi-hua（Department of Infectious Diseases， Tangdu Hospital， the Fourth Military Medical University， Xi’an 710038， China）

Abstract：ObjectiveTo construct recombinant plasmids of pCR3.1-S encoding protein S of hepatitis B virus as nucleic acid vaccines and investigate the effects of recombinant human interleukin 2 as adjuvants on immune responses induced by HBV nucleic acid vaccines in BALB／c mice. Methods Anti-HBs antibodies to HBV major protein was measured by ELISA assay， and the activities of lymphocyte proliferation and cytotoxity were detected by³H-TdR incorporation assay and 4-hour ⁵¹Cr release assay. Humoral and cellular immune responses of different groups were then compared. Results The titers of anti-HBs antibodies in serum of rhIL-2 group mice were not significantly higher than thoseof control group mice （P＞0.05）． The difference of the activities of lymphocyte proliferation and cytotoxity between of control group mice and rhIL-2 group mice （every mouse with 500 U one time） was not significant （P＞0.05）． However， stimulation index and specific lysis rate of lymphocyte of rhIL-2 group mice （every mouse with 1000 U or 2000 U one time） showed significant difference compared with htoseof control group mice （P＜0.05）． Conclusion Recombinant plasmids of pCR3.1-S as HBV nucleic acid vaccines could induced strong humoral and cellular immune responses in BALB／c mice. rhIL-2 acts as adjuvants to increase the potency of HBV nucleic acid vaccine in some certainty.

Keywords：interleukin 2； hepatitis B virus； nucleic acid vaccine基金项目：国家自然科学基金资助项目（39770665）

作者简介：李文波（1974－）男，山西洪洞县人，硕士，住院医师，主要从事乙型肝炎病毒核酸疫苗的研究。Tel：（0531）2187681-66066；E-mail：wenboligood@263.net参考文献：

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收稿日期：2000年3月20日

修稿日期：2000年9月27日

出版日期：2001年1月15日