THE RELATIONSHIP BETWEEN BIOMECHANICAL PROPERTIES OF DEEP FROZEN CRYOPRESERVATING ALLOGRAFT AND THE PRESERVATING TIME
yang Ke-Qiang,Wang Zhen,Sang Hong-Xun(Department of Orthopedics, 255th Hospital of PLA, TANG SHAN, HEBEI, 063000)
Abstract:Objective: To study the relationship between the preservating time of deep frozen cryopreservating allograft and the biomechanical properties. Method: Specimens of the fibular bones of deep frozen cryopreservation for 1 and 7 years were made into test pieces of torture and compaction. And the bending strength(BS), modulus of elasticity(ME) and compact strength(CS) were measured on the INSTRON1195 electronic tensile test machine. The results were analysised with T test. Results: The 1 year group and the 7 year group had no significant difference in terms of BS (P>0.05), ME(P>0.05) and CS(P>0.05). Conclusion: Cryopreservation had no offects on the cortical bone biomechanics, as long as not being contaminated, the cryopreservation will be independent of storage time and the pre-or pro-operation treatments.
Keywords:Deep Frozen Bone, Biomechanics, Allografts作者简介:杨克强 第四军医大学骨科研究所部队临床医学中青年人才培养基金班学员参考文献:
[1]Finn HA. Technical Aspects and surgical complication of segmental allografts Following segmental resection of maligmant bone tumors. In: Langlais F. Tomenoed B. Limb salvage-major reconstructions in oncologic and nontumoral conditions.Verlag Berlin:Springer Heidelberg, 1991, 75~87.
[2]Mizutani H, and Urist MR, The nature of bone morphogenetic protein(BMP)fractions derived from bovine bone matrix gelatin, Clin orthop, 1982; 171: 213.
[3]任祖翰,骨与软骨的低温保存.见:刘金刚,低温医学.北京,人民卫生出版社.1993: 213.
[4]郭仁棣,张宏民,杨育勇.国人尺骨静态弹性范围内力学性的测定.中国生物医学工程学报.1994; 13(1): 74-77.
[5]Komender A. Infiuence of preservation on soms mechanical propertis of human haversian bone. Mater Med Pol 1976; 8: 13.
[6]Pelker RR. Friedlaender GD. Markham TC. Biomechanical properties of bone allografts. Clin Orthop 1983; 174: 54.
[7]Ma ZG, Zhou SX, Liu BL.The mechanical and biological properties of deminera-liged cortical bone allografts in animals. J Bone Joint Surg (Br), 1991, 73: 791.
[8]Urist MR. Bone formation in implants of partially and wholly demincralized bone matrix. Clin othop, 1970, 71: 27.
[9]Tomford WW, Doppelt SH, Mankin HJ, et al. Bone bank procedures. Clin orthop. 1983; 174: 15.
