关键词: 人肝增强因子，发酵，大肠杆菌

摘要：目的：研究表达重组人肝增强因子(hALR)工程菌JMl09的发酵工艺。方法：采用发酵罐发酵，优化工程菌发酵的培养基配方、pH值、诱导表达时间、补料方式等。结果：在pH6.9条件下，培养6h后诱导表达5h，同时补料，5L罐发酵工程菌，菌体收得量可达湿重33.0g／L。目标蛋白表达量约占菌体总蛋白的31.3％。结论：此发酵工艺可以较好地提高ALR工程菌菌体得率和目标蛋白表达量。

分类号：R978

Study on the Fermentation Process of E. cloi JM109 Expressing Human Augmenter of Liver Regenration

li Rubing,Shan Zhongyuan,Fu Yonghang,Yang Lianping,Yi Xuerui,Kong Xiangping(Department of Molecular Biology, Infectious Disease Center, the 458thHospital of PLA Guangzhou 510602)

Abstract：Purpose: To develop the best fermentation process on E. coli JM109 expressing human augmenter of liver regenration (hALR). Methods: Optimizing the composition of the medium,the range of pH, induction time and the way of fed-batch in fermentation tank. The expression of recombinant protein was analyzed. Stable fermentation parameters were obtained. Results: Under the established conditions,33.0g of wet bacteria per liter could be obtained, and the derivative of recombinant hALR was about 31.3％ of total protein in the host. Conclusion: The developed fermentative technology might increase the collection ratio and expression of recombinant hALR.

Keywords：Recombinant hALR, Fermentation, E. coli参考文献：

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收稿日期：1999-07-28