关键词: 表皮生长因子，活性肽，细胞培养，肝坏死，小牛

摘要：目的：研究小牛类表皮生长因子活性肽(C-epidermalgrowthfactor-likeactivepeptide,C-EGF)在体内外对肝细胞再生的影响。方法：从小牛颌下腺提取C-EGF，利用细胞培养及CCl4诱导的大鼠肝坏死模型，检测了C-EGF对肝细胞再生的刺激作用。结果：C-EGF可促进人肝癌细胞系SMMC-7721细胞及小鼠胎肝细胞的增殖；在肝坏死动物模型中，C-EGF可显著地启动并促进肝细胞的再生，C-EGF对细胞增殖的刺激作用在体内外均可被胰岛素增强。结论：C-EGF在体内外对肝细胞的增殖均有促进作用，此结果为C-EGF在肝病治疗中的应用提供了实验依据。

Effect of Calf-epidermal Growth Factor-like Active Peptide on Hepatocyte Proliferation

li Suqin,Tang Guozhi,Li Faqin,Tan Weiguo,Jin Huiying,Fang Dexing(Institute of Military Medicine,Nanjing Command,PLA (Nanjing 210002))

Abstract：To study the effects of calf-epidermal growth factor-like active peptide(C-EGF)on the proliferation of hepatocytes in vivo and in vitro.Methods:C-EGF was isolated from calf submaxillary gland.By means of cell culture and model of rat liver necrosis,the effects of C-EGF on the proliferation of hepatocytes were studied.Results:C-EGF could stimulate the proliferation of SMMC-7721 hepatoma cells and increase the colonies o'fetal mouse hepatocytes in half-solid culture.In model of liver necrosis induced by CCl4,C-EGF can initiate and stimulate regeneration of hepatocytes distinctively.The effect of C-EGF on stimulating proliferation could be enhanced by insulin both in vivo and in vitro.Conclution:C-EGF enhances the proliferation of hypatocytes both in vivo and in vitro.These results provide an experimental basis for the application of C-EGF in the treatment of liver diseases.

Keywords：Calf-epidermal growth factor-like active peptide,Active peptide,Cell culture,Liver necrosis,Calf

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收稿日期：1999-04-19

修稿日期：1999-08-18 2000-01-05