关键词: 附子，中性多糖，酸性蛋白多糖

摘要：目的：探讨黄附子中的糖复合物，为深入研究和开发利用附子提供有用资料。方法：采用热水抽提、乙醇沉淀、DEAE-C32及SephadexG75柱层析等方法分离黄附子中的糖复合物，用酚-硫酸法、Folin-酚法及硫酸-咔唑法测定其糖、蛋白质、葡萄糖醛酸的含量。结果：从黄附子中得到两种糖复合物，一种为中性多糖，含量为3％；另一种为酸性蛋白多糖，含量为1％(其中糖含量97％、蛋白质含量3％、葡萄糖醛酸含量9％)。结论：黄附子中两种糖复合物的获得有助于研究附子抗癌、抗衰老和增强免疫机能的作用。

Isolation,Purification and Characterization of the Neutral Polysaccharide and Acidic Proteinpolysaccharide of Aconitum Carmichaeli Debx

ruan Qiping,Zhou Li,Zhao Li(Department of Biology,Mianyang Teachers’College(Mianyang 621000))

Abstract：To detect those complex carbohydrates in aconitum carmichaeli debx for getting the useful data.Methods:Two complex carbohydrates were isolated from aconitum carmichaeli debx by conventional methods,including distilled water extraction,ethanol precipitation,DEAE-C32 and Sephadex G75 column chromatography.The contents of sugar,protein and glucuronic acid in them were determined by Phenol-vitriol,Folin-phenol and Vitriol-carbazole methods.Results:Between two complex carbohydrates obtained from aconitum carmichaeli debx,one was the neutral polysaccharidethe and the content of it was 3％.another was the acidic proteinpolysaccharide and the content of it was 1％,and the contents of total sugar,protein and glucuronic acid were 97％,3％ and 9％ respctively.Conclusion:Two complex carbohydrates from aconitum carmichaeli debx might be conducive to the investigation on the effectiveness of anti-cancer,anti-aging and enhancing the immune function of aconitum carmichaeli debx.

Keywords：Aconitum carmichaeli debx,Neutral polysaccharide,Acidic proteinpolysaccharide

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收稿日期：1999-05-27

修稿日期：1999-07-27 1999-10-16