关键词: 紫杉醇，7-表-紫杉醇，层析，氧化铝催化

摘要：目的：研究在氧化铝层析初分离红豆杉浸膏中紫杉醇增量的来源。方法：采用C18-硅胶反相层析、氧化铝层析及硅胶层析等方法对物料进行处理，HPLC分析紫杉醇的含量。结果：紫杉醇增量主要来源于红豆杉浸膏中7-表-紫杉醇的碱性氧化铝催化下的异构化。结论：优化7-表-紫杉醇的异构化条件，可以增加7-表-紫杉醇向紫杉醇的转化。

Studies of Taxol Convertible from Taxus Extract Catalyzed with Al2O3

zhang Zhiqiang,Su Zhiguo(Institute of Microbiolgy,Chinese Acaden, of Sciences(Beijing 100080))

Abstract：To study the resource of the increased of taxol during the Al2O3 chromatography to initial separation of taxol from Taxus Yunnanensis extract.Methods:The extract was treated with Cl8-silica gel reversed-phase chromatography,Al2O3 chromatography and silica gel chromatography.The content of taxol was determined by HPLC.Results:The main resource of the increased taxol was the isomerization of 7-epi-taxol under the catalysis of Al2O3.Conclusion:7-epi-taxol could be more convertible to taxol under the optimized conditions of the isomeric reaction.

Keywords：Taxol,7-epi-taxol,Chromatography,Catalysis of Al2O3基金项目：国家自然科学基金重点项目(项目号：29736180)参考文献：

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收稿日期：1999-08-09

修稿日期：1999-10-08