摘要：根据猪繁殖与呼吸综合征病毒(PRRSV)美洲型膜蛋白和核衣壳蛋白基因序列，设计了一对含有EcoR I和BamH I酶切位点的引物，用RT-PCR对四个流产猪场的病料进行了检测，扩增出约918 bp的基因片段。通过病毒分离、酶切鉴定和序列分析证实为PRRSV感染。结果说明应用所设计的引物进行RT-PCR快速检测PRRS是可行的，为我国快速特异诊断PRRS和PRRSV强毒株的深入研究奠定了基础。

中图分类号：S858.28文献标识码：A

文章编号：1003-5125(2000)03-0272-05

Direct Detection of Porcine Reproductive and Respiratory

Syndrome Virus of Stillborn Fetus Tissues by Reverse

Transcription Polymerase Chain Reaction (RT-PCR)

CAI Jia-li

(Animal Husbandry and Veterinary Medical College,Southwest Agricultural University, Chongqing 400716, China)

CAI Bao-xiang，JIANG Ping

(Animal Medical College, Nanjing Ariculture University, Nanjing 210095, China)

Abstract: Primers for RT-PCR were designed on the basis of the M and N gene sequences of U. S. isolate of PRRSV. The primers have restriction endonuclease (EcoR I and BamH I) sites. The tissues of stillborn fetuses of affected swine were detected by RT-PCR in 4 pig farms. The samples were RT-PCR positive. A gene fragment about 918 bp was amplified by RT-PCR, PRRSV were isolated from the tissues of stillborn fetuses. The result showed that PRRSV can be quickly diagnosed by RT-PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.

Key words: PRRS; RT-PCR: Detection; Diagnosis

作者简介：蔡家利(1956年-)，男，重庆市人，副教授，博士，主要研究动物病毒性传染病

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收稿日期：1999-04-08，修回日期：1999-06-15