摘要：对蜀柏毒蛾核型多角体病毒(Parocneria orienta Nuclear polyhedrovirus,简称PaorNPV)形态结构、结构多肽、限制性内切酶图谱等特性进行了研究。采用不连续系统垂直板SDS-PAGE分析了PaorNPV的多角体蛋白、病毒粒子结构多肽。应用5种限制性内切酶对PaorNPV基因组DNA进行了酶切分析。结果表明：经热处理的多角体蛋白仅有一条带，分子量为31.5 kD，不经热处理的多角体蛋白有三条带，分子量分别为31.5 kD、29.1 kD、28.6 kD；病毒粒子包含有25种结构多肽，分子量范围在17.6-114.6 kD之间。PaorNPV DNA经BamH I。EcoR I、HindⅢ、Pst I和Xho I酶切分别产生9、12、12、12和14条片段。基因组大小平均为124.6 kb。

中图分类号：S763.42文献标识码：A

文章编号：1003-5125(2000)03-0249-06

Analysis of Structural Polypeptides and Restriction Patterns

of Parocneria orienta Nuclear polyhedrovirus

ZHAO Xiao-dong，DING Qing-quan

(Wuhan Institute of Virology, Academia Sinica, Wuhan 430071)

ZHOU Jian-hua

(Sichuan Academy of Forest, Chendu 610081)

Abstract: The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS-PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, BamH I, EcoR I, Hind Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb). Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).

Key words: Parocneria orienta Nuclear polyhedrovirus (PaorNPV), Structural polypeptides, Restriction endonucleas pattern

第一作者简介：赵小东(1972年-)，男，湖北黄冈人，在读研究生，研究方向：分子病毒学。

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收稿日期：1999-07-23，修回日期：2000-02-24